Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47298 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD223 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 µg/mL PHA for 5 days were stained with Goat Anti-Human LAG-3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47298) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody and Mouse Anti-Human CD3ε PE-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining.