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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-76403 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal LAG-3 Antibody
- Antibody type
- Monoclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 2 - 8 C 1 year from date of receipt
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T lysate. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate. Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) and rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate. Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) and rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR028F) [NBP2-76403] - Section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.
