A700-027

antibody from Invitrogen Antibodies

Targeting: LAG3 CD223

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-027

Provider

Invitrogen Antibodies

Product name

LAG3 Recombinant Rabbit Monoclonal Antibody (BLR027F)

Antibody type

Monoclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Antibody clone number

BLR027F

Concentration

0.50 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate. Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027) and rabbit anti-LAG3 recombinant monoclonal [BLR028F] A700-028. Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ [G11], and 293T lysate. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH (A300-641A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (Product # A700-027 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (Product # A700-027 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LAG3 in FFPE tonsil by IHC.Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027), Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Lag3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-Lag3 recombinant monoclonal antibody [BLR027F] (Product #A700-027) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).