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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-806TS-T100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-806TS-T100, RRID:AB_11181040
- Product name
- LAG-3 (human) monoclonal antibody (17B4) (ATTO 647N conjugate)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Purified from concentrated hybridoma tissue culture supernatant by Protein A Sepharoseª CL-4B Flow Fast Column.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 17B4
- Vial size
- 100 tests
- Storage
- +4°C
- Handling
- Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light.
Submitted references CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells.
Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation.
A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors.
The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells.
Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway.
T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts.
CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling.
Characterization of the major histocompatibility complex class II binding site on LAG-3 protein.
Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand.
Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens.
Boer MC, van Meijgaarden KE, Joosten SA, Ottenhoff TH
PloS one 2014;9(4):e94192
PloS one 2014;9(4):e94192
Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation.
Casati C, Camisaschi C, Novellino L, Mazzocchi A, Triebel F, Rivoltini L, Parmiani G, Castelli C
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3782-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):3782-8
A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors.
Triebel F, Hacene K, Pichon MF
Cancer letters 2006 Apr 8;235(1):147-53
Cancer letters 2006 Apr 8;235(1):147-53
The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells.
Maçon-Lemaître L, Triebel F
Immunology 2005 Jun;115(2):170-8
Immunology 2005 Jun;115(2):170-8
Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway.
Di Carlo E, Cappello P, Sorrentino C, D'Antuono T, Pellicciotta A, Giovarelli M, Forni G, Musiani P, Triebel F
The Journal of pathology 2005 Jan;205(1):82-91
The Journal of pathology 2005 Jan;205(1):82-91
T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts.
Demeure CE, Wolfers J, Martin-Garcia N, Gaulard P, Triebel F
European journal of cancer (Oxford, England : 1990) 2001 Sep;37(13):1709-18
European journal of cancer (Oxford, England : 1990) 2001 Sep;37(13):1709-18
CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling.
Hannier S, Tournier M, Bismuth G, Triebel F
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):4058-65
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):4058-65
Characterization of the major histocompatibility complex class II binding site on LAG-3 protein.
Huard B, Mastrangeli R, Prigent P, Bruniquel D, Donini S, El-Tayar N, Maigret B, Dréano M, Triebel F
Proceedings of the National Academy of Sciences of the United States of America 1997 May 27;94(11):5744-9
Proceedings of the National Academy of Sciences of the United States of America 1997 May 27;94(11):5744-9
Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand.
Huard B, Gaulard P, Faure F, Hercend T, Triebel F
Immunogenetics 1994;39(3):213-7
Immunogenetics 1994;39(3):213-7
Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens.
Baixeras E, Huard B, Miossec C, Jitsukawa S, Martin M, Hercend T, Auffray C, Triebel F, Piatier-Tonneau D
The Journal of experimental medicine 1992 Aug 1;176(2):327-37
The Journal of experimental medicine 1992 Aug 1;176(2):327-37
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of endogenous human LAG-3 by FACS analysis using LAG-3 (human), mAb (17B4) (ATTO 647) (Prod. No. ALX-804-806TS)Method: Human PBMC were stimulated (B) or not (A) with 1µg/ml of superantigen SEB. After 2 days PBMC were stained with 10µg/ml (1µg/0.5x10E6 cells) of LAG-3 (human), mAb (17B4) (ATTO 647) (Prod. No. ALX-804-806TS) and analyzed by flow cytometry.
