Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- BAF2319 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LAG-3 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LAG-3 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P18627
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LAG-3 in CD3+ Human PBMCs by Flow Cytometry. CD3+ Human peripheral blood mononuclear cells (PBMCs) treated with 5 μg/mL PHA for 5 days were stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Goat Anti-Human LAG-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2319) or (B) Normal Goat IgG Biotinylated Control (Catalog # BAF108) followed by Streptavidin-Allophycocyanin (Catalog # F0050).