Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB8039 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HOIP/RNF31 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human HOIP/RNF31 in direct ELISA and Western Blot.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function.
Douglas T, Saleh M
Cell reports 2019 Dec 10;29(11):3652-3663.e5
Cell reports 2019 Dec 10;29(11):3652-3663.e5
Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function.
Zhu F, Yi G, Liu X, Zhu F, Zhao A, Wang A, Zhu R, Chen Z, Zhao B, Fang S, Yu X, Lin R, Liang R, Li D, Zhao W, Zhang Z, Guo W, Zhang S, Ge S, Fan X, Zhao G, Li B
The Journal of biological chemistry 2018 Dec 28;293(52):20099-20111
The Journal of biological chemistry 2018 Dec 28;293(52):20099-20111
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human HOIP/RNF31 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog # MAB8039) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HOIP/RNF31 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human HOIP/RNF31 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for HOIP/RNF31 at approximately 144 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog # MAB8039). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.