Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-1094 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1094, RRID:AB_2180947
- Product name
- Goat Polyclonal HOIP/RNF31 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Roles of c-IAP proteins in TNF receptor family activation of NF-κB signaling.
Immunoaffinity enrichment coupled to quantitative mass spectrometry reveals ubiquitin-mediated signaling events.
Ubiquitination profiling identifies sensitivity factors for IAP antagonist treatment.
TRIADs: a new class of proteins with a novel cysteine-rich signature.
Varfolomeev E, Goncharov T, Vucic D
Methods in molecular biology (Clifton, N.J.) 2015;1280:269-82
Methods in molecular biology (Clifton, N.J.) 2015;1280:269-82
Immunoaffinity enrichment coupled to quantitative mass spectrometry reveals ubiquitin-mediated signaling events.
Yu K, Phu L, Varfolomeev E, Bustos D, Vucic D, Kirkpatrick DS
Journal of molecular biology 2015 Jun 5;427(11):2121-34
Journal of molecular biology 2015 Jun 5;427(11):2121-34
Ubiquitination profiling identifies sensitivity factors for IAP antagonist treatment.
Varfolomeev E, Izrael-Tomasevic A, Yu K, Bustos D, Goncharov T, Belmont LD, Masselot A, Bakalarski CE, Kirkpatrick DS, Vucic D
The Biochemical journal 2015 Feb 15;466(1):45-54
The Biochemical journal 2015 Feb 15;466(1):45-54
TRIADs: a new class of proteins with a novel cysteine-rich signature.
van der Reijden BA, Erpelinck-Verschueren CA, Löwenberg B, Jansen JH
Protein science : a publication of the Protein Society 1999 Jul;8(7):1557-61
Protein science : a publication of the Protein Society 1999 Jul;8(7):1557-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HOIP/RNF31 Antibody [NB100-1094] - Staining using this antibody (2ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HOIP/RNF31 Antibody [NB100-1094] - (0.5ug/ml) staining of JURKAT lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.