HPA039337

antibody from Atlas Antibodies

Targeting: POLR1D AC19, MGC9850, RPA16, RPA9, RPAC2, RPO1-3

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA039337

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA039337, RRID:AB_10672961

Product name

Anti-POLR1D

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human POLR1D, Gene description: polymerase (RNA) I polypeptide D, 16kDa, Alternative Gene Names: MGC9850, RPA16, RPA9, RPAC2, RPO1-3, Validated applications: IHC, ICC, WB, Uniprot ID: Q9Y2S0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation

Yang G, Xin Q, Feng I, Wu D, Dean J
Genes & Development 2023;37(9-10):418-431

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and POLR1D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407348).

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.

Sample type

Human