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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-52754 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MIGNMEHMTA GGNCQTFSSS TVISYSNTGD GAPKVYQETS EMRSAPGGIR ETRRTVRDSD SGLEQMSIGH HIRDRAHILQ RSRNHRTGDQ EERQDYINLD ESEAAAFDDE WRRETSRFRQ QRPLEFRRLE SSGA
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MLF2 in control (vector only transfected HEK293T lysate) and MLF2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MLF2 Polyclonal Antibody (Product # PA5-52754).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MLF2 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MLF2 Polyclonal Antibody (Product # PA5-52754).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human liver using MLF2 Polyclonal Antibody (Product # PA5-52754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human kidney using MLF2 Polyclonal Antibody (Product # PA5-52754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human testis using MLF2 Polyclonal Antibody (Product # PA5-52754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human cerebral cortex tissue shows strong nuclear and cytoplasmic positivity in neuronal cells while strong cytoplasmic positivity in glial cells. Samples were probed using a MLF2 Polyclonal Antibody (Product # PA5-52754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human cerebral cortex and pancreas tissues using MLF2 Polyclonal Antibody (Product # PA5-52754). Corresponding MLF2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human cerebral cortex, kidney, liver and testis using MLF2 Polyclonal Antibody (Product # PA5-52754) (A) shows similar protein distribution across tissues to an independent MLF2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MLF2 in human pancreas using MLF2 Polyclonal Antibody (Product # PA5-52754) shows low expression as expected.
