MA5-50460

antibody from Invitrogen Antibodies

Targeting: RPLP2 D11S2243E, LP2, MGC71408, P2, RPP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50460

Provider

Invitrogen Antibodies

Product name

RPLP2 Recombinant Rabbit Monoclonal Antibody (HL2256)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

HL2256

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) on 293T (30 μg), A431 (30 μg), HeLa (30 μg), and HepG2 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 15% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Rat kidney tissue extract (50 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Whole zebrafish extract (30 μg) using a dilution of 1:20,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in A431 cells at a dilution of 1:500. Cells were fixed in ice-cold MeOH for 5 min. Green: RPLP2 stained by RPLP2 antibody ( MA5-50460) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded human colon tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded mouse stomach, brain, lung, and testis tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded human colon tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.