Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- MA5-50460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPLP2 Recombinant Rabbit Monoclonal Antibody (HL2256)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL2256
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) on 293T (30 μg), A431 (30 μg), HeLa (30 μg), and HepG2 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 15% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Rat kidney tissue extract (50 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Whole zebrafish extract (30 μg) using a dilution of 1:20,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in A431 cells at a dilution of 1:500. Cells were fixed in ice-cold MeOH for 5 min. Green: RPLP2 stained by RPLP2 antibody ( MA5-50460) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded human colon tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded mouse stomach, brain, lung, and testis tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPLP2 using RPLP2 Recombinant Monoclonal Antibody (Product # MA5-50460) in Paraffin embedded human colon tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.