Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- IC2419P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PDX-1/IPF1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse PDX-1/IPF1 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references In vitro quantitative and relative gene expression analysis of pancreatic transcription factors Pdx-1, Ngn-3, Isl-1, Pax-4, Pax-6 and Nkx-6.1 in trans-differentiated human hepatic progenitors.
Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
Vishwakarma SK, Rahamathulla S, Bardia A, Tiwari SK, Srinivas G, Raj A, Tripura C, Sandhya A, Habeeb MA, Khan AA, Pande G, Reddy KP, Reddy PY
Journal of diabetes investigation 2014 Sep;5(5):492-500
Journal of diabetes investigation 2014 Sep;5(5):492-500
Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
Kornete M, Beauchemin H, Polychronakos C, Piccirillo CA
Autoimmunity 2013 Jun;46(4):259-68
Autoimmunity 2013 Jun;46(4):259-68
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of PDX-1/IPF1 in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of PDX-1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry. BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.