TA324981

antibody from OriGene

Targeting: PDX1 IDX-1, IPF1, MODY4, PDX-1, STF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324981

Provider

OriGene

Product name

Rabbit polyclonal IPF Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal IPF Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

PDX1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Ipf Antibody (C-term) (Cat. #TA324981) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of U251 cell stained with IPF Antibody (C-term)(Cat#TA324981).U251 cells were incubated with IPF primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Validation comment

IF