GTX81323

antibody from GeneTex

Targeting: PDX1 IDX-1, IPF1, MODY4, PDX-1, STF-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81323

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81323, RRID:AB_11165962

Product name

PDX1 antibody, C-term

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Ipf Antibody (C-term) (GTX81323) western blot analysis in U-937 cell line lysates (35μg/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term)(GTX81323).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.