Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX81323 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81323, RRID:AB_11165962
- Product name
- PDX1 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Ipf Antibody (C-term) (GTX81323) western blot analysis in U-937 cell line lysates (35μg/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term)(GTX81323).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.