Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-6500-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Pdx1 Monoclonal Antibody (2A12), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 2A12 monoclonal antibody reacts with the transcriptional activator PDX1, also known as pancreatic and duodenal homeobox protein and insulin promoter factor. Early expression of PDX1 during development marks the endoderm from which the pancreas, stomach, and duodenum derive. In the mature pancreas, expression is found in insulin-expressing beta-cells. PDX1 functions as an important regulator of several key pancreatic proteins including insulin, somatostatin, glucokinase, islet amyloid factor, and glucose transporter type 2. Deficiency in PDX1 results in a dysregulation of the beta-cell compensation of insulin resistance, which can lead to late-onset type 2 diabetes mellitus (formerly NIDDM) and maturity onset diabetes of the young type 4 (MODY4). Applications Reported: This 2A12 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 2A12 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)) and can be used at less than or equal to 5 ug/mL. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A12
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Pancreatic duodenal homeobox 1 protein is a novel beta-cell-specific autoantigen for type I diabetes.
Autophagy regulates pancreatic beta cell death in response to Pdx1 deficiency and nutrient deprivation.
Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress.
Li SW, Koya V, Li Y, Donelan W, Lin P, Reeves WH, Yang LJ
Laboratory investigation; a journal of technical methods and pathology 2010 Jan;90(1):31-9
Laboratory investigation; a journal of technical methods and pathology 2010 Jan;90(1):31-9
Autophagy regulates pancreatic beta cell death in response to Pdx1 deficiency and nutrient deprivation.
Fujimoto K, Hanson PT, Tran H, Ford EL, Han Z, Johnson JD, Schmidt RE, Green KG, Wice BM, Polonsky KS
The Journal of biological chemistry 2009 Oct 2;284(40):27664-73
The Journal of biological chemistry 2009 Oct 2;284(40):27664-73
Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress.
Sachdeva MM, Claiborn KC, Khoo C, Yang J, Groff DN, Mirmira RG, Stoffers DA
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 10;106(45):19090-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 10;106(45):19090-5
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human pancreas using 5 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL Anti-Human PDX1 Purified (right) followed by Anti-Mouse IgG HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.