Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 50-6500-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Pdx1 Monoclonal Antibody (2A12), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 2A12 monoclonal antibody reacts with the transcriptional activator PDX1, also known as pancreatic and duodenal homeobox protein and insulin promoter factor. Early expression of PDX1 during development marks the endoderm from which the pancreas, stomach, and duodenum derive. In the mature pancreas, expression is found in insulin-expressing beta-cells. PDX1 functions as an important regulator of several key pancreatic proteins including insulin, somatostatin, glucokinase, islet amyloid factor, and glucose transporter type 2. Deficiency in PDX1 results in a dysregulation of the beta-cell compensation of insulin resistance, which can lead to late-onset type 2 diabetes mellitus (formerly NIDDM) and maturity onset diabetes of the young type 4 (MODY4). Applications Reported: This 2A12 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This 2A12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A12
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human pancreas stained with 20 µg/mL Mouse IgG1 K Isotype Control eFluor® 660 (left) or 20 µg/mL Anti-Human PDX1 eFluor® 660 (right). Nuclei are stained with DAPI.