PA5-13046

antibody from Invitrogen Antibodies

Targeting: PDX1 IDX-1, IPF1, MODY4, PDX-1, STF-1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13046

Provider

Invitrogen Antibodies

Product name

Phospho-PDX1 (Thr11) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Deficiency in catechol-o-methyltransferase is linked to a disruption of glucose homeostasis in mice.

Kanasaki M, Srivastava SP, Yang F, Xu L, Kudoh S, Kitada M, Ueki N, Kim H, Li J, Takeda S, Kanasaki K, Koya D
Scientific reports 2017 Aug 11;7(1):7927

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot blot analysis of Phospho-IPF pThr11 polyclonal antibody (Product # PA5-13046) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 2-ME induced insulin secretion in MIN6 cells. ( a ) Time-dependent insulin secretion by 2-ME (5, 50, 100 and 500 nM concentrations) at 3 mM, 6 mM and 30 mM medium glucose concentrations. Insulin estimation by the ELISA method performed in triplicate. Three sets of independent experiments were performed. ( b , c ) Western blot data analysis of control and 2-ME-treated MIN6 cells. A representative image of 5 blots is shown. Cropped images were displayed and original blots are shown in the figure Supplementary 14 . Densitometry data were normalized to beta-actin. ( d ) Time-dependent insulin secretion by 2-ME in the scramble and AMPK siRNA transfected MIN6 cells. Scramble and AMPK siRNA were transfected using lipofectamine 2000 at 100 nM concentration in cells. Insulin estimation was performed in triplicate. Three sets of independent experiments were analyzed. ( e ) Western blot analysis of total AMPK and AMPK phosphorylation protein levels in the scramble and AMPK siRNA transfected MIN6 cells. A representative image from 5 blots is shown. Cropped images were displayed and original blots are shown in the figure Supplementary 14 . Densitometry data normalized to beta-actin. ( f ) Western blot analysis of total AMPK and AMPK phosphorylation after treatment with 2-ME and AICAR at 3 mM glucose and 30 mM glucose concentration. A representative image from 4 blots is shown. Cropped images were displayed and original blots are shown in the figure Supplementary 15 . ( g ) Tim