Explore
Validate
LearnPA5-17436
antibody from Invitrogen Antibodies
Targeting: PNPLA2
ATGL, desnutrin, FP17548, iPLA2zeta, TTS-2.2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-17436 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATGL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 142 µg/mL
- Storage
- -20°C
Submitted references Carboxyl Ester Lipase May Not Mediate Lipotoxic Injury during Severe Acute Pancreatitis.
Khatua B, Trivedi RN, Noel P, Patel K, Singh R, de Oliveira C, Trivedi S, Mishra V, Lowe M, Singh VP
The American journal of pathology 2019 Jun;189(6):1226-1240
The American journal of pathology 2019 Jun;189(6):1226-1240
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATGL in extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells using ATGL polyclonal antibody (Product # PA5-17436).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using ATGL Polyclonal Antibody (Product # PA5-17436) and a 55 kDa band corresponding to ATGL was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Raw 264.7 (Lane 1), NIH: OVCAR-3 (Lane 2), NIH/3T3 (Lane 3), Mouse adipose (Lane 4), Mouse skeletal muscle (Lane 5), 3T3-L1 (Lane 6), 3T3-L1 differentiated to adipocytes (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATGL in 3T3-L1 cells using an ATGL polyclonal antibody (Product # PA5-17436) (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATGL in 3T3-L1 cells using an ATGL polyclonal antibody (Product # PA5-17436) (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATGL Polyclonal Antibody (Product # PA5-17436) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to vacuolar structure (fat droplets) in cytoplasm. Panel e shows 3T3-L1 cells with less expression of ATGL. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
