PA5-18661

antibody from Invitrogen Antibodies

Targeting: PNPLA2 ATGL, desnutrin, FP17548, iPLA2zeta, TTS-2.2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18661

Provider

Invitrogen Antibodies

Product name

ATGL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human Adipose lysate using Product # PA5-18661 at a concentration of 0.2 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using ATGL Polyclonal Antibody (Product # PA5-18661) and a 52 kDa band corresponding to ATGL was observed in adipose tissue only when compared to skeletal muscle. Whole cell extracts (30 µg lysate) of Rat adipose (Lane 1), Rat skeletal muscle (Lane 2) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).