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LearnPA5-29064
antibody from Invitrogen Antibodies
Targeting: PNPLA2
ATGL, desnutrin, FP17548, iPLA2zeta, TTS-2.2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
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- Product number
- PA5-29064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATGL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, mouse white adipose. Predicted reactivity: Mouse (83%), Rat (83%), Pig (80%), Sheep (85%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PNPLA2 using 50 µg of mouse white adipose tissue. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PNPLA2 polyclonal antibody (Product # PA5-29064) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PNPLA2 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PNPLA2 polyclonal antibody (Product # PA5-29064) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATGL was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATGL Polyclonal Antibody (Product # PA5-29064) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATGL was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATGL Polyclonal Antibody (Product # PA5-29064) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
