R30870

antibody from NSJ Bioreagents

Targeting: SPTLC1 hLCB1, HSAN1, HSN1, LCB1, SPTI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30870

Provider

NSJ Bioreagents

Product name

SPTLC1 Antibody / Serine palmitoyltransferase 1

Antibody type

Polyclonal

Description

This highly specific SPTLC1 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the SPTLC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of SPTLC1 antibody and Lane 1: SW620; 2: S549; 3: PANC; 4: U87 cell lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human SiHa, 2) human U-251, 3) rat brain and 4) mouse brain tissue lysate with SPTLC1 antibody. Expected molecular weight ~53 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: SPTLC1 antibody testing of human breast cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer tissue with SPTLC1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.