Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 15334-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15334-1-AP, RRID:AB_10858229
- Product name
- POLR2F antibody
- Antibody type
- Polyclonal
- Description
- POLR2F antibody (Cat. #15334-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors.
Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.
Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.
Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.
Wang H, Li B, Zuo L, Wang B, Yan Y, Tian K, Zhou R, Wang C, Chen X, Jiang Y, Zheng H, Qin F, Zhang B, Yu Y, Liu CP, Xu Y, Gao J, Qi Z, Deng W, Ji X
Nature communications 2022 Sep 28;13(1):5703
Nature communications 2022 Sep 28;13(1):5703
Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.
Li Y, Huang J, Zhu J, Bao L, Wang H, Jiang Y, Tian K, Wang R, Zheng H, Duan W, Lai W, Yi X, Zhu Y, Guo T, Ji X
Molecular cell 2022 Oct 20;82(20):3943-3959.e11
Molecular cell 2022 Oct 20;82(20):3943-3959.e11
Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.
Jiang Y, Huang J, Tian K, Yi X, Zheng H, Zhu Y, Guo T, Ji X
Genome biology 2022 Nov 28;23(1):246
Genome biology 2022 Nov 28;23(1):246
Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.
Ke F, Yu XD, Wang ZH, Gui JF, Zhang QY
Cell & bioscience 2022 Jan 6;12(1):6
Cell & bioscience 2022 Jan 6;12(1):6
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP(POLR2F antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using POLR2F antibody 15334-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 15334-1-AP(POLR2F antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 15334-1-AP(POLR2F antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue