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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-51462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: GDDFDDVEED EGLDDLENAE EEGQENVEIL PSGERPQANQ KRITTPYMTK YERARVLGTR ALQIAMCAPV MVELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELII Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR2F in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR2F in human cell line U-251 MG shows positivity in nucleus & nucleoli. Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of POLR2F in human cell line U-251 MG using POLR2F Polyclonal Antibody (Product # PA5-51462). Staining shows localization to nucleus and nucleoli fibrillar center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of POLR2F in human cerebellum using POLR2F Polyclonal Antibody (Product # PA5-51462) shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of POLR2F in human cerebellum using POLR2F Polyclonal Antibody (Product # PA5-51462) shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
