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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-51462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GDDFDDVEED EGLDDLENAE EEGQENVEIL PSGERPQANQ KRITTPYMTK YERARVLGTR ALQIAMCAPV MVELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELII
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR2F in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR2F in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR2F in human cell line U-251 MG shows positivity in nucleus & nucleoli. Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR2F in human cerebellum tissue shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer. Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
