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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-27546 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, MCF-7, MDA-MB-231. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Xenopus laevis (95%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract. B. 30 µg MDA-MB-231 whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPS15 in paraformaldehyde-fixed MCF-7 cells using a RPS15 polyclonal antibody (Product # PA5-27546) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
