Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
Reference
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- Product number
- ABIN500728 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Spindle and Kinetochore Associated Complex Subunit 1 (SKA1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of Human SKA1.
- Description
- Peptide Afiinity Chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3.
Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
Influence of an extrinsic cross-link on the folding pathway of ribonuclease A. Conformational and thermodynamic analysis of cross-linked (lysine7-lysine41)-ribonuclease a.
Gaitanos TN, Santamaria A, Jeyaprakash AA, Wang B, Conti E, Nigg EA
The EMBO journal 2009 May 20;28(10):1442-52
The EMBO journal 2009 May 20;28(10):1442-52
Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.
Hanisch A, Silljé HH, Nigg EA
The EMBO journal 2006 Nov 29;25(23):5504-15
The EMBO journal 2006 Nov 29;25(23):5504-15
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
Cleveland DW, Mao Y, Sullivan KF
Cell 2003 Feb 21;112(4):407-21
Cell 2003 Feb 21;112(4):407-21
Influence of an extrinsic cross-link on the folding pathway of ribonuclease A. Conformational and thermodynamic analysis of cross-linked (lysine7-lysine41)-ribonuclease a.
Lin SH, Konishi Y, Denton ME, Scheraga HA
Biochemistry 1984 Nov 6;23(23):5504-12
Biochemistry 1984 Nov 6;23(23):5504-12
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at 0.5 μg/ml (A) and 1 ?g/ml (B).