Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-83557 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83557, RRID:AB_11022832
- Product name
- Rabbit Polyclonal GRPEL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GRPEL1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Redox regulation of GRPEL2 nucleotide exchange factor for mitochondrial HSP70 chaperone.
Konovalova S, Liu X, Manjunath P, Baral S, Neupane N, Hilander T, Yang Y, Balboa D, Terzioglu M, Euro L, Varjosalo M, Tyynismaa H
Redox biology 2018 Oct;19:37-45
Redox biology 2018 Oct;19:37-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRPEL1 Antibody [NBP1-83557] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRPEL1 Antibody [NBP1-83557] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRPEL1 Antibody [NBP1-83557] - Analysis in human cell lines PC-3 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRPEL1 Antibody [NBP1-83557] - Staining of human duodenum shows strong cytoplasmic positivity, with a granular pattern in glandular cells.