PA5-56975

antibody from Invitrogen Antibodies

Targeting: SLC12A6 ACCPN, KCC3

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56975

Provider

Invitrogen Antibodies

Product name

KCC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RVRFSSRESV PETSRSEPMS EMSGATTSLA TVALDPPSDR TSHPQDVIED LSQNSITGEH SQLLDDGHKK ARNAYLNNSN YEEGDE Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 93%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.

Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences 2020 Jun 3;10(6)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of KCC3 in human cell line A-431 shows positivity in cytoplasm & vesicles. Samples were probed using a KCC3 Polyclonal Antibody (Product # PA5-56975).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Quantification of KCC3/GABA A R clustering on iDNs and mouse DRG neurons. ( A , B ) Both day 40 iDNs and mouse DRG neurons stained positive for KCC3-IR and GABA A R labeling (arrow heads neurite selections). Line scan analyses on 20 um neurite structures were performed. ( A ) Lower intensity levels of GABA A R clustering in iDNs were elucidated as compared with mouse DRGs ( B ). 2-3 individual differentiations, scale bars 20 um and 10 um (enlargement), line scan was set to 20 um.