Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF427 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Flt-3 Ligand/FLT3L Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Flt-3 Ligand/FLT3L in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Flt-3 Ligand/FLT3L is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response.
Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism.
Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Hillyer LM, Woodward B
Cytokine 2019 Jan;113:39-49
Cytokine 2019 Jan;113:39-49
Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism.
Bertho JM, Kereselidze D, Manens L, Culeux C, Magneron V, Surette J, Blimkie M, Bertrand L, Wyatt H, Souidi M, Dublineau I, Priest N, Jourdain JR
Scientific reports 2019 Dec 27;9(1):19919
Scientific reports 2019 Dec 27;9(1):19919
Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
Proceedings of the National Academy of Sciences of the United States of America 2010 May 25;107(21):9759-64
Proceedings of the National Academy of Sciences of the United States of America 2010 May 25;107(21):9759-64
Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
Blumenthal RD, Reising A, Leon E, Goldenberg DM
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 May;8(5):1301-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 May;8(5):1301-9
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Shih CC, Hu MC, Hu J, Weng Y, Yazaki PJ, Medeiros J, Forman SJ
Blood 2000 Mar 15;95(6):1957-66
Blood 2000 Mar 15;95(6):1957-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Flt-3 Ligand and Neutralization by Mouse Flt-3 Ligand Antibody. Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Flt-3 Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Flt-3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427). The ND50 is typically 0.05-0.25 µg/mL.