Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF2467 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Flt-3 Ligand/FLT3L Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects feline Flt-3 Ligand/FLT3L in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human Flt-3 Ligand/FLT3L and recombinant mouse Flt-3 Ligand/FLT3L is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by Flt-3 Ligand and Neutral-ization by Feline Flt-3 Ligand Antibody. Recombinant Feline Flt-3 Ligand (Catalog # 2467-FL) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline Flt-3 Ligand (2 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline Flt-3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467). The ND50 is typically 0.1-0.4 µg/mL.