H00002323-M07

antibody from Abnova Corporation

Targeting: FLT3LG

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002323-M07

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002323-M07, RRID:AB_1111845

Product name

FLT3LG monoclonal antibody (M07), clone 4C4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant FLT3LG.

Antigen sequence

TQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVA
SNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQG
LLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLL
QETSE


Isotype

IgG

Antibody clone number

4C4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FLT3LG is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between KITLG and FLT3LG. HeLa cells were stained with anti-KITLG rabbit purified polyclonal 1:1200 and anti-FLT3LG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)