MAB78791

antibody from Novus Biologicals

Targeting: GLG1 CFR-1, ESL-1, MG-160

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB78791

Provider

Novus Biologicals

Product name

Mouse Monoclonal Golgi Glycoprotein 1/GLG1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Golgi Glycoprotein 1/GLG1 in direct ELISA. Detects human and mouse GLG1 in Western Blots.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.