A305-733A-T

antibody from Invitrogen Antibodies

Targeting: CHMP7 MGC29816

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-733A-T

Provider

Invitrogen Antibodies

Product name

CHMP7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHMP7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHMP7 antibody (Product # A305-733A-T; lot A305-733A-T-2) used for WB at 1:1,000.Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHMP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHMP7 antibody A305-733A-M (lot A305-733A-M-1) used for IP at 20 µl per reaction. CHMP7 was also immunoprecipitated by rabbit anti-CHMP7 antibody A305-734A-M. For blotting immunoprecipitated CHMP7, A305-733A-M was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHMP7 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHMP7 antibody A305-733A-M (lot A305-733A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHMP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHMP7 antibody (Product # A305-733A-T (lot A305-733A-T-2) used for IP at 20 µL per reaction. CHMP7 was also immunoprecipitated by a previous lot of this antibody (lot A305-733A-T-1). For blotting immunoprecipitated CHMP7, Product # A305-733A-T was used at 1:1,000. Detection: Chemiluminescence with an exposure time of 30 seconds.