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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-253-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-5R alpha/CD125 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-5 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL-1 RII, rhIL-2 R beta , rhIL-2 R gamma , rhIL-3 R is observed and less than 1% cross-reactivity with rhIL-1 RI, rhIL-4 R, rhIL-6 R, rhIL-7 R, rhIL-9 R, and rhIL-10 R is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Three lysine residues in the common β chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling.
Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Lei JT, Mazumdar T, Martinez-Moczygemba M
The Journal of biological chemistry 2011 Nov 18;286(46):40091-103
The Journal of biological chemistry 2011 Nov 18;286(46):40091-103
Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
Lei JT, Martinez-Moczygemba M
Journal of leukocyte biology 2008 Aug;84(2):499-509
Journal of leukocyte biology 2008 Aug;84(2):499-509
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Human IL-5 R alpha/CD125 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-5 R alpha/CD125 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-253-NA). The ND50 is typically 1-3 µg/mL.
