Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47340 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL5RA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL-1 RII, rhIL-2 R beta, rhIL-2 R gamma, rhIL-3 R is observed and less than 1% cross-reactivity with rhIL-1 RI, rhIL-4 R, rhIL-6 R, rhIL-7 R, rhIL-9 R, and rhIL-10 R is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL5RA in TF‚1 human erythroleukemic cell line. Samples were incubated in IL5RA polyclonal antibody (Product # PA5-47340). Recombinant Human IL‚5 stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚5 Rα/CD125 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1-3 µg/mL.