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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- FAB253A - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-5 R alpha/CD125 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-5 R alpha/CD125 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) IL-5 R beta , rhIL-9 R, rhIL-4 R, rmIL-5 R alpha , or rhIL-13 R alpha 1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 26815
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-5 R alpha/CD125 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human IL-5 R alpha/CD125 APC-conjugated Monoclonal Antibody (Catalog # FAB253A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-5 R alpha/CD125 in HEK293 Human Cell Line Transfected with Human IL-5 R alpha/CD125 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human IL-5 R alpha/CD125 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human IL-5 R alpha/CD125 APC-conjugated Monoclonal Antibody (Catalog # FAB253A). Quadrant markers were based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
