Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX630779 - Provider product page
- Provider
- GeneTex
- Product name
- Triosephosphate isomerase antibody [GT181]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg A431 whole cell extract C. 30 £gg HeLa whole cell extract D. 30 £gg HepG2 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg Neuro2A whole cell extractB. 30 £gg C8D30 whole cell extractC. 30 £gg NIH-3T3 whole cell extractD. 30 £gg Raw264.7 whole cell extractE. 30 £gg C2C12 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg PC-12 whole cell extractB. 30 £gg Rat2 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.