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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-18291 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPI1 Monoclonal Antibody (P181)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P181
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPI1 Monoclonal Antibody (P181) detects Triosephosphate isomerase protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg A431 whole cell extract. C. 30 µg HeLa whole cell extract. D. 30 µg HepG2 whole cell extract.12 % SDS-PAGE. TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPI1 Monoclonal Antibody (P181) detects Triosephosphate isomerase protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg A431 whole cell extract. C. 30 µg HeLa whole cell extract. D. 30 µg HepG2 whole cell extract.12 % SDS-PAGE. TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPI1 Monoclonal Antibody (P181) detects Triosephosphate isomerase protein by western blot analysis. A. 30 µg Neuro2A whole cell extract. B. 30 µg C8D30 whole cell extract. C. 30 µg NIH-3T3 whole cell extract. D. 30 µg Raw264.7 whole cell extract. E. 30 µg C2C12 whole cell extract.12 % SDS-PAGE. TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPI1 Monoclonal Antibody (P181) detects Triosephosphate isomerase protein by western blot analysis. A. 30 µg PC-12 whole cell extract. B. 30 µg Rat2 whole cell extract.12 % SDS-PAGE. TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPI1 Monoclonal Antibody (P181) detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase protein stained by TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TPI1 was performed in paraffin-embedded mouse testis tissue using TPI1 Monoclonal Antibody (P181) (Product # MA5-18291) at a dilution of 1:100.
