- PA5-18342 - Invitrogen Antibodies TPI1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot staining of Human liver lysate using Product # PA5-18342 at a concentration of 0.003 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot staining of Human liver lysate using Product # PA5-18342 at a concentration of 0.003 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), A549 (Lane 4), C2C12 (Lane 5), MOLT-4 (Lane 6), RAW 264.7 (Lane 7), U-87 MG (Lane 8), tissue extracts of Mouse Skeletal Muscle (Lane 9) and Mouse Liver (Lane 10). The blot was probed with Anti-TPI1 Polyclonal Antibody (Product # PA5-18342, 0.1 µg/ml) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution). A 31 kDa band corresponding to TPI1 was observed across cell lines and tissues tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of TPI1 was achieved by transfecting Hep G2 cells with TPI1 specific siRNAs (Silencer® select Product # s224747). Western blot analysis (Fig. a) was performed using whole cell extracts from the TPI1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TPI1 Polyclonal Antibody (Product # PA5-18342, 0.1µg/ml) and Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TPI1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TPI1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TPI1 Polyclonal Antibody (Product # PA5-18342) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TPI1 in paraffin embedded human liver using a TPI1 polyclonal antibody (Product #PA5-18342) at a concentration of 2 µg/mL. Steamed antigen retrieval was performed with pH 9 buffered Tris and EDTA. Samples were then stained with horseradish peroxidase.

PA5-18342