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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504888 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504888, RRID:AB_2622676
- Product name
- C20orf30 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)
- Antibody type
- Monoclonal
- Description
- C20orf30 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TMEM230
- Isotype
- IgG
- Antibody clone number
- OTI2C8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Identification of TMEM230 mutations in familial Parkinson's disease.
Deng HX, Shi Y, Yang Y, Ahmeti KB, Miller N, Huang C, Cheng L, Zhai H, Deng S, Nuytemans K, Corbett NJ, Kim MJ, Deng H, Tang B, Yang Z, Xu Y, Chan P, Huang B, Gao XP, Song Z, Liu Z, Fecto F, Siddique N, Foroud T, Jankovic J, Ghetti B, Nicholson DA, Krainc D, Melen O, Vance JM, Pericak-Vance MA, Ma YC, Rajput AH, Siddique T
Nature genetics 2016 Jul;48(7):733-9
Nature genetics 2016 Jul;48(7):733-9
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf30 (RC201878, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf30.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-C20orf30 mouse monoclonal antibody (TA504888) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C20orf30(RC201878).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201878 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C20orf30 antibody(TA504888), and then analyzed by flow cytometry.
- Validation comment
- FC
