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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06719PU-N - Provider product page
- Provider
- OriGene
- Product name
- NEDD8 mouse monoclonal antibody, clone 5B8, Purified
- Antibody type
- Monoclonal
- Description
- NEDD8 mouse monoclonal antibody, clone 5B8, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEDD8
- Isotype
- IgG
- Antibody clone number
- 5B8
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 antibody against human NEDD8 (AA: 1-81) recombinant protein (Expected MW is 40 kDa).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 antibody against C6 (1) and Hela (2) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using NEDD8 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 antibody with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Hela cells using NEDD8 antibody (green) and negative control (red).
- Validation comment
- FC
