Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN2845525 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32), (N-Term) antibody
- Antibody type
- Polyclonal
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 1-32, N-Term
- Antibody clone number
- RB0639
- Vial size
- 80 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast cancer cells.
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1.
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases.
Fan M, Bigsby RM, Nephew KP
Molecular endocrinology (Baltimore, Md.) 2003 Mar;17(3):356-65
Molecular endocrinology (Baltimore, Md.) 2003 Mar;17(3):356-65
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA
Nature 2003 Mar 20;422(6929):330-4
Nature 2003 Mar 20;422(6929):330-4
Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Bohnsack RN, Haas AL
The Journal of biological chemistry 2003 Jul 18;278(29):26823-30
The Journal of biological chemistry 2003 Jul 18;278(29):26823-30
DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1.
Wu K, Yamoah K, Dolios G, Gan-Erdene T, Tan P, Chen A, Lee CG, Wei N, Wilkinson KD, Wang R, Pan ZQ
The Journal of biological chemistry 2003 Aug 1;278(31):28882-91
The Journal of biological chemistry 2003 Aug 1;278(31):28882-91
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases.
Liu J, Furukawa M, Matsumoto T, Xiong Y
Molecular cell 2002 Dec;10(6):1511-8
Molecular cell 2002 Dec;10(6):1511-8
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC