ALX-210-194-R200

antibody from Enzo Life Sciences

Targeting: NEDD8 Nedd-8

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ALX-210-194-R200

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-210-194-R200, RRID:AB_2051982

Product name

NEDD8 polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

200 μl

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis.

Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, Lu Y, Liu P, Li Y, Wang S, Chai N, Wu J, Deng H, Wang HR, Cao Y, Zhao F, Cui Y, Wang J, He F, Zhang L
Nature communications 2014 May 13;5:3733

---

Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes.

Hjerpe R, Thomas Y, Chen J, Zemla A, Curran S, Shpiro N, Dick LR, Kurz T
The Biochemical journal 2012 Feb 1;441(3):927-36

---

The essential functions of NEDD8 are mediated via distinct surface regions, and not by polyneddylation in Schizosaccharomyces pombe.

Girdwood D, Xirodimas DP, Gordon C
PloS one 2011;6(5):e20089

---

Hypoxia-inducible factors in the first trimester human lung.

Groenman F, Rutter M, Caniggia I, Tibboel D, Post M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2007 Apr;55(4):355-63

---

The COP9 signalosome regulates Skp2 levels and proliferation of human cells.

Denti S, Fernandez-Sanchez ME, Rogge L, Bianchi E
The Journal of biological chemistry 2006 Oct 27;281(43):32188-96

---

Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity.

Xirodimas DP, Saville MK, Bourdon JC, Hay RT, Lane DP
Cell 2004 Jul 9;118(1):83-97

---

Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha.

Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, Pierce JW, Podust VN, Luo RS, Chau V, Palombella VJ
Molecular and cellular biology 2000 Apr;20(7):2326-33

---

Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity of SCF(skp2) toward p27(kip1).

Morimoto M, Nishida T, Honda R, Yasuda H
Biochemical and biophysical research communications 2000 Apr 21;270(3):1093-6

---

Cleavage of the C-terminus of NEDD8 by UCH-L3.

Wada H, Kito K, Caskey LS, Yeh ET, Kamitani T
Biochemical and biophysical research communications 1998 Oct 29;251(3):688-92

---

Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein.

Kamitani T, Kito K, Nguyen HP, Yeh ET
The Journal of biological chemistry 1997 Nov 7;272(45):28557-62