14258-1-AP

antibody from Proteintech Group

Targeting: MBD3

Provider product page for 14258-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14258-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14258-1-AP, RRID:AB_2139745
Product name: MBD3 antibody
Antibody type: Polyclonal
Description: KD/KO validated MBD3 antibody (Cat. #14258-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MBD3 protein structure - 14258-1-AP shown in red.

Submitted references HP1 enhances radio- and chemotherapy resistance in osteosarcoma via PARP1-dependent transcriptional repression and HR repair.

Zhu Y, Li W, Qiu Y, Deng Z, Li Y, Chen H, Zhang J, Lu D, Hu K, Huang D, Yin D
Cancer letters 2026 Feb 28;639:218213

Inactivation of TACC2 epigenetically represses CDKN1A and confers sensitivity to CDK inhibitors.

Lin ZR, Xia TL, Wang MY, Zhang LJ, Liu YM, Yuan BY, Zhou AJ, Yuan L, Zheng J, Bei JX, Lin DX, Zeng MS, Zhong Q
Med (New York, N.Y.) 2025 May 9;6(5):100568

Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma.

Yang J, Lu X, Cai Q, Liu M, Xia T, Hong D, Le L, Zhang X, Zhang X
Molecular cancer 2025 May 30;24(1):158

Nuclear Translocation of S100A9 Triggers Senescence of Human Amnion Fibroblasts by De-Repressing LINE1 Via Heterochromatin Erosion at Parturition.

Zhang F, Li MD, Pan F, Lei WJ, Xi Y, Ling LJ, Myatt L, Sun K, Wang WS
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jun;12(21):e2414682

Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation.

Li P, Hu X, Fan Z, Sun S, Ran Q, Wei T, Wei P, Jiang Q, Yan J, Yang N, Jia C, Yang T, Mao Y, Cai X, Xu T, Zhao Z, Qian X, Qin W, Zhuang X, Fan F, Xiao J, Zheng Z, Li S
Journal of hematology & oncology 2024 Sep 2;17(1):77

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development.

Chen X, Huang MF, Fan DM, He YH, Zhang WJ, Ding JC, Peng BL, Pan X, Liu Y, Du J, Li Y, Liu ZY, Xie BL, Kuang ZJ, Yi J, Liu W
Nucleic acids research 2024 Jul 8;52(12):6811-6829

MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway.

Wang H, Min J, Ding Y, Yu Z, Zhou Y, Wang S, Gong A, Xu M
Biological procedures online 2024 Jan 5;26(1):1

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells.

Dai W, Liu Z, Yan M, Nian X, Hong F, Zhou Z, Wang C, Fu X, Li X, Jiang M, Zhu Y, Huang Q, Lu X, Hou L, Yan N, Wang Q, Hu J, Mo W, Zhang X, Zhang L
Developmental cell 2024 Feb 26;59(4):482-495.e6

MBD3 as a Potential Biomarker for Colon Cancer: Implications for Epithelial-Mesenchymal Transition (EMT) Pathways.

Ding Y, Wang H, Liu J, Jiang H, Gong A, Xu M
Cancers 2023 Jun 14;15(12)

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Wang XH, Xu S, Zhou XY, Zhao R, Lin Y, Cao J, Zang WD, Tao H, Xu W, Li MQ, Zhao SM, Jin LP, Zhao JY
Nature communications 2021 Jun 8;12(1):3428

Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation.

Zhan X, Guo S, Li Y, Ran H, Huang H, Mi L, Wu J, Wang X, Xiao D, Chen L, Li D, Zhang S, Yan X, Yu Y, Li T, Han Q, He K, Cui J, Li T, Zhou T, Rich JN, Bao S, Zhang X, Li A, Man J
The Journal of experimental medicine 2020 May 4;217(5)

Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.

Li S, Yang H, Zhao M, Gong L, Wang Y, Lv Z, Quan Y, Wang Z
Oncology letters 2020 Dec;20(6):280

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Liang MR, Zeng Y, Zeng SY, Zhang JW, Yang BC, Zhang ZY, Liu FY, Luo Y, Zou Y, Wang F, Huang OP
Genetic testing and molecular biomarkers 2019 Aug;23(8):523-532

Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.

Ma P, Pan H, Montgomery RL, Olson EN, Schultz RM
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):E481-9

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP(MBD3 antibody) at dilution of 1:600
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human endometrial cancer using 14258-1-AP(MBD3 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human endometrial cancer using 14258-1-AP(MBD3 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue