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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347241 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal MBD3 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal MBD3 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MBD3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with the ab at 1:2,000 in TBS-Tween with 5% milk. B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An ab against the TY1 tag or the ab against MBD3 (1:2,000 in TBS-Tween & 5% milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer.To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human MBD3. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.
- Validation comment
- ELISA
