PAB14055

antibody from Abnova Corporation

Targeting: MBD3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB14055

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB14055, RRID:AB_10552042

Product name

MBD3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MBD3.

Storage

Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U-2 OS).Nuclear extracts were prepared and separated by electrophoresis.An antibody directed against the TY1 tag (Lane 1) or crude serum (Lane 2) MBD3 polyclonal antibody (Cat # PAB14055) (diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U-937 NE, 60 ug) with MBD3 polyclonal antibody (Cat # PAB14055), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ELISA was performed using a serial dilution of MBD3 polyclonal antibody (Cat # PAB14055) in antigen coated wells. By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 13,890.

Validation comment

ELISA