691702

antibody from BioLegend

Targeting: TUBA8 TUBAL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

691702

Provider

BioLegend

Proper citation

BioLegend Cat#691702, RRID:AB_2632654

Product name

Purified anti-Tubulin α 8

Antibody type

Monoclonal

Description

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta tubulin. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. Tubulin α 8 belongs to the Tubulin α family which contains 8 other members such as tubulin α 1A, tubulin α 4A, tubulin α 3E. It has been shown that mutations in the tubulin α 8 gene is associated with polymicrogyria and optic nerve hypoplasia.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

P82D6D7

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from HeLa (lane 1, 15 µg), MCF-7 (lane 2, 15 µg) and Jurkat (lane 3, 15 µg) were resolved by electrophoresis (4-12 % Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Tubulin α 8 antibody (clone P82D6D7). Proteins were visualized using a HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Purified anti-β-actin antibody (clone Poly6221) was used as a loading control.

Conjugate

Unconjugated