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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- CF501038 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUBA8 Monoclonal Antibody (OTI2E9), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2E9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8. Positive lysates LY412867 (100 µg) and LC412867 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8. Positive lysates LY412867 (100 µg) and LC412867 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-TUBA8 mouse monoclonal antibody (TA501038) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8(RC211175).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY TUBA8(RC211175)(Red) or empty vector control plasmid (Blue) were immunostained with anti-TUBA8 mouse monoclonal(TA501038), and then analyzed by flow cytometry.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of TUBA8 by using TrueMab monoclonal anti-TUBA8 antibodies (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
