PA5-110555

antibody from Invitrogen Antibodies

Targeting: USP18

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110555

Provider

Invitrogen Antibodies

Product name

USP18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: RPRAWDYPHG LVGLHNIGQ

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

3.08 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP18 in various lysates. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of USP18 in A431 cells. Samples were incubated in polyclonal USP18 antibody (Product # PA5-110555) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of USP18 in C6 cells. Samples were incubated in polyclonal USP18 antibody (Product # PA5-110555) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of USP18 in L929 cells. Samples were incubated in polyclonal USP18 antibody (Product # PA5-110555) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of USP18 in MCF7 cells. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded rat brain. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded human colon carcinoma. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded human colon. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded mouse spinal cord. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded rat brain. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded human colon carcinoma. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP18 in paraffin-embedded human colon. Samples were incubated with USP18 Polyclonal antibody (Product # PA5-110555) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.