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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7117 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7117, RRID:AB_1571140
- Product name
- ALOX5AP polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ALOX5AP.
- Antigen sequence
C-HKVEHESRTQNGR- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ALOX5AP expression, but not gene haplotypes, is associated with obesity and insulin resistance.
Kaaman M, Rydén M, Axelsson T, Nordström E, Sicard A, Bouloumié A, Langin D, Arner P, Dahlman I
International journal of obesity (2005) 2006 Mar;30(3):447-52
International journal of obesity (2005) 2006 Mar;30(3):447-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ALOX5AP polyclonal antibody (Cat # PAB7117) (0.1 ug/mL) staining of human U-937 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ALOX5AP polyclonal antibody (Cat # PAB7117)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
