PA5-77793

antibody from Invitrogen Antibodies

Targeting: HSPH1 HSP105A, HSP105B, KIAA0201, NY-CO-25

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77793

Provider

Invitrogen Antibodies

Product name

HSP105 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunohistochemistry samples may be prepared using Bouin's Fixative solution. 1 µg/mL of PA5-77793 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 110kDa.

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Yeast

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation.

Calle A, Toribio V, Yáñez-Mó M, Ramírez MÁ
International journal of molecular sciences 2021 May 26;22(11)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 BBT secret implantation proteins on EVs, and their secretion pattern, which is altered after the uptake of MSC-EVs or the presence of uterine cytokines. SEC-elution profile of EVs from BBT-9 and BBT-18 for the different experimental conditions (Control, Activin A, Activin A+FLST, pbMSC-EVs, tau-pbMSC-EVs, eMSC-EVs, and tau-eMSC-EVs) were analyzed by Dot Blot using an anti-CD9 specific antibody. The positive EV fractions of each experimental condition were quantified by ImageJ software analysis of the dot blot results ( a ). Bead-assisted flow cytometry analysis of selected implantation proteins expressed in BBT-9- ( b ) and BBT-18-EVs ( c ) after cell stimulation.