MA5-36231

antibody from Invitrogen Antibodies

Targeting: HSPH1 HSP105A, HSP105B, KIAA0201, NY-CO-25

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-36231

Provider

Invitrogen Antibodies

Product name

HSPH1 Monoclonal Antibody (3D10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human Caco-2 whole cell, human K562 whole cell, human A549 whole cell, human HepG2 whole cell, human PANC-1 whole cell, human SGC-7901 whole cell. IHC: human lung cancer tissue, human mammary cancer tissue, human intestinal cancer tissue. ICC/IF: A431 cell, MCF7 cell. Flow: A431 cell, HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3D10

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSPH1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSPH1 in A431 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSPH1 in A431 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human mammary cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in HepG2 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in A431 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in HepG2 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in A431 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).